Procedures High-resolution imaging

  1. Ensure the Ronchigram is perfectly blown up.


  1. Increase the magnification in the TEM center to above ~ 50Mx.


  1. Locate a rough focus range:
    Adjust the Focus knob until you see (when crossover) the lattice fringes orientate in one direction and
    the lattice fringes orientate in another direction (90 degree).

4. Fine focus optimization and fine A1 optimization
Adjust the Focus knob and the Fine A1 (Hardware: COND STIG + Corse mode ) iteratively.
>> When the atoms are focused, they should be very bright for a very short moment.
>> When astigmatisms are removed, the lattice fringes disappear and become individual distinctive atoms.

 Then, adjust the focus to another crossover
Adjust the Focus knob and the Fine A1 (Hardware: COND STIG + Corse mode ) iteratively.
>> When the atoms are focused, they should be very bright for a very short moment.
>> When astigmatisms are removed, the lattice fringes disappear and become individual distinctive atoms.

5. Capture the image in


Procedures of Drift Correction and Image Stacking

  1. B >> DigitalScanStack >> Capture Stacks: 40 images (slice through 40 images) >> you can see the slide number

If the sample does not drift much, you may stack 100 pieces of images together.
If the sample keeps drifting we shall then stack fewer images (30 pieces of image) together.




  • If the sample does not drift much, you may stack 100 pieces of images together. Of course, if the sample keeps drifting we shall then stack fewer images (30 pieces of image) together.

  • B >> DigitalScanStack >> Capture Stacks: 40 images (slice through 40 images) >> you can see the slide number

  • Home >> Process Image >> Image Alignment >> Measure >> Remove (shift x and y to offset the shfit)

  • Process >> Scale >> Scale Image

  • Volume >> Project >> Project Image >> Along Z direction

Capture = slow scan